5LPU
Crystal structure of Annexin A2 complexed with S100A4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-06 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.740, 61.240, 148.160 |
Unit cell angles | 90.00, 88.69, 90.00 |
Refinement procedure
Resolution | 49.374 - 2.100 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hyw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.683 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.374 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 75455 | |
<I/σ(I)> | 23.1 | 2.42 |
Completeness [%] | 100.0 | 99.87 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1M Bis Tris pH 6.5, 0.2M ammonium acetate 4% PEG4000 and 1,5% glycerol |