5LP7
Crystal structure of 3-Ketoacyl-CoA Thiolase (MmgA) from Bacillus subtilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.180, 141.120, 211.462 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 31.920 - 2.200 |
| R-factor | 0.15282 |
| Rwork | 0.150 |
| R-free | 0.20461 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.293 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.910 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.097 | 0.410 |
| Number of reflections | 161184 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M sodium iodide, 0.1 M BTP pH 8.5, 20 % PEG 3350 |
