5LOV

DZ-2384 tubulin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	103.160, 154.700, 180.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.350 - 2.400
R-factor	0.1911
Rwork	0.188
R-free	0.24150
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.004
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.350	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.110	4.070
Number of reflections	112935
<I/σ(I)>	18.6	0.75
Completeness [%]	99.8	99.7
Redundancy	13.6	13.5
CC(1/2)	1.000	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	293	4-10% PEG 4000, 4-6% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 100 mM MES/Imidazole