5LOE
Structure of full length Cody from Bacillus subtilis in complex with Ile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 134.690, 158.877, 55.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.450 - 3.000 |
| R-factor | 0.21693 |
| Rwork | 0.212 |
| R-free | 0.30162 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b18 2b0l |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.065 | 0.654 |
| Number of reflections | 22830 | |
| <I/σ(I)> | 25.3 | 0.96 |
| Completeness [%] | 92.5 | 47.3 |
| Redundancy | 5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15% PEG5000, 0.1M HEPES pH 7.5, 5% tacsimate, 20mM Ile |
