5LNW

Crystal structure of Arabidopsis thaliana Pdx1-I320-G3P complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97525
Spacegroup name	H 3
Unit cell lengths	178.261, 178.261, 115.309
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.565 - 1.900
R-factor	0.1826
Rwork	0.181
R-free	0.21950
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.934
Data scaling software	Aimless (0.5.15)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.570	44.570	1.930
High resolution limit [Å]	1.900	10.410	1.900
Rmerge	0.123	0.144	0.861
Rmeas	0.145	0.168	1.047
Rpim	0.074	0.083	0.585
Total number of observations	345059	2074	15929
Number of reflections	107077
<I/σ(I)>	6.1	13.2	1.3
Completeness [%]	99.4	95.2	97.8
Redundancy	3.2	3.3	3
CC(1/2)	0.977	0.946	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris pH8.5, 0.2 M sodium acetate pH5.5, 10%(w/v) PEG4000