5LNH
Structure of full length Unliganded CodY from Bacillus subtilis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97820 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.869, 110.550, 257.414 |
Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
Resolution | 46.680 - 3.000 |
R-factor | 0.23279 |
Rwork | 0.230 |
R-free | 0.27936 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gx5 2b0l |
RMSD bond length | 0.018 |
RMSD bond angle | 1.607 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.102 | |
Number of reflections | 57358 | |
<I/σ(I)> | 18.3 | 0.51 |
Completeness [%] | 72.0 | |
Redundancy | 3.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M sodium citrate pH 5.6, 20 mM Tris-HCl pH 7.5, 35 % ammonium sulphate |