5LNH
Structure of full length Unliganded CodY from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97820 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.869, 110.550, 257.414 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 46.680 - 3.000 |
| R-factor | 0.23279 |
| Rwork | 0.230 |
| R-free | 0.27936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gx5 2b0l |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.607 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.102 | |
| Number of reflections | 57358 | |
| <I/σ(I)> | 18.3 | 0.51 |
| Completeness [%] | 72.0 | |
| Redundancy | 3.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M sodium citrate pH 5.6, 20 mM Tris-HCl pH 7.5, 35 % ammonium sulphate |
