5LMX
Monomeric RNA polymerase I at 4.9 A resolution
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Number of Particle | 190750 |
| Software | RELION,CTFFIND,UCSF Chimera,PHENIX |
| CTF correction | PHASE FLIPPING ONLY |
