5LMK
Structure of phopsho-CDK2-cyclin A in complex with an ATP-competitive inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.466, 134.395, 149.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.940 - 2.400 |
| R-factor | 0.21333 |
| Rwork | 0.212 |
| R-free | 0.24863 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CDK2?cyclin A structure |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.950 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 253641 |
| <I/σ(I)> | 13.2 |
| Completeness [%] | 98.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | ammonium sulphate; potassium chloride; sodium Hepes pH7.5 |
