5LMF
Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator bound to Dcp2 HLM3 peptide (region 484-500)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.360, 122.920, 126.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.480 - 2.150 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ogp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 41458 | |
| <I/σ(I)> | 7.5 | 0.94 |
| Completeness [%] | 99.6 | 93.9 |
| Redundancy | 3 | |
| CC(1/2) | 0.459 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297.15 | 0.2 M MgCl2 ; 20% PEG3350 |
