5LK5

Crystal structure of the globular domain of human calreticulin mutant D71K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 42
Unit cell lengths	196.976, 196.976, 67.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.770 - 2.300
R-factor	0.21211
Rwork	0.210
R-free	0.24685
RMSD bond length	0.016
RMSD bond angle	1.688
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.280
Number of reflections	114120
<I/σ(I)>	9.41
Completeness [%]	95.8
Redundancy	4.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	30% PEG4000 0.2 M MgCl2 0.1 M Tris-HCl