5LHY

PB3 Domain of Human PLK4 (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	220.350, 220.350, 325.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.367 - 3.310
R-factor	0.2705
Rwork	0.269
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Chainsaw model based on 1MBY
RMSD bond length	0.002
RMSD bond angle	0.528
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.180
High resolution limit [Å]	3.310	3.310
Rmerge		1.353
Number of reflections	119458
<I/σ(I)>	16.6
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 nl protein solution (52.3 mg/ml protein in 20 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT) and 100 nl mother liquor (32.27% v/v PPG400, 100 mM NaCl, 50 mM MgCl2, unbuffered)