5LHD

Structure of glycosylated human aminopeptidase N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	127.093, 168.856, 244.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.973 - 2.600
R-factor	0.1831
Rwork	0.181
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.779
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Rmerge	0.050
Number of reflections	160764
<I/σ(I)>	12.3
Completeness [%]	99.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	50 mM Imidazol-HCl pH 8.0, 20% PEG6000