5LEX

Native human 20S proteasome in Mg-Acetate at 2.2 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	113.860, 203.150, 316.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	170.890 - 2.200
R-factor	0.17922
Rwork	0.177
R-free	0.22140
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5le5
RMSD bond length	0.016
RMSD bond angle	1.755
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	170.890	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	346869
<I/σ(I)>	13	0.9
Completeness [%]	98.8	99.4
Redundancy	6.9
CC(1/2)	1.000	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris, 0.2 M Magnesium Chloride, 10 % PEG3350