5LCD
Structure of Polyphosphate Kinase from Meiothermus ruber bound to AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000010 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 164.441, 164.441, 95.044 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 116.280 - 2.660 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lc9 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | autoPROC (1.0.5) |
Data scaling software | Aimless (0.5.14) |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.280 | 2.680 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.322 | 2.089 |
Rmeas | 0.329 | |
Rpim | 0.049 | |
Total number of observations | 1661253 | |
Number of reflections | 37790 | |
<I/σ(I)> | 17.3 | 2.4 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 44 | 31.2 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 298 | PEG3350, Li2SO4 |