5LC1
L-threonine dehydrogenase from Trypanosoma brucei with NAD and the inhibitor pyruvate bound.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.074 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 132.040, 276.490, 55.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.500 - 2.100 |
| R-factor | 0.14952 |
| Rwork | 0.147 |
| R-free | 0.20763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l9a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.794 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.500 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.145 | 0.525 |
| Number of reflections | 117204 | |
| <I/σ(I)> | 11.4 | 3.2 |
| Completeness [%] | 97.3 | 81.9 |
| Redundancy | 7.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 20 % w/v PEG 10K; TDH 2.0 mg/ml, 1 mM NAD(+), 30 mM pyruvate. |
