5LB4
Apo-structure of humanised RadA-mutant humRadA14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97903 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.511, 37.814, 69.451 |
Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
Resolution | 33.710 - 1.980 |
R-factor | 0.23 |
Rwork | 0.229 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4a6p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.100 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.052 | 0.700 |
Number of reflections | 14305 | |
<I/σ(I)> | 20.51 | 2.29 |
Completeness [%] | 99.1 | 98.1 |
Redundancy | 3.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 25% PEG4000, 0.1 M Tris pH 8.5 |