5LB4
Apo-structure of humanised RadA-mutant humRadA14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.511, 37.814, 69.451 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 33.710 - 1.980 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a6p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.052 | 0.700 |
| Number of reflections | 14305 | |
| <I/σ(I)> | 20.51 | 2.29 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 25% PEG4000, 0.1 M Tris pH 8.5 |
