5LAJ
Ligand-induced Lys33-Thr1 crosslinking at the yeast proteasomal subunit beta5 by sulfonate esters
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 137.250, 300.530, 146.420 |
Unit cell angles | 90.00, 113.73, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.18152 |
Rwork | 0.180 |
R-free | 0.20097 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cz4 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.912 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.080 | 0.535 |
Number of reflections | 231423 | |
<I/σ(I)> | 10.6 | 2.6 |
Completeness [%] | 96.6 | 98 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MGAC2, 13% MPD |