5L9W
Crystal structure of the Apc core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 240.080, 240.080, 336.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.971 - 2.900 |
| R-factor | 0.1985 |
| Rwork | 0.197 |
| R-free | 0.23510 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmeas | 0.117 | 2.420 |
| Number of reflections | 121963 | |
| <I/σ(I)> | 25 | 1.7 |
| Completeness [%] | 96.4 | 90.5 |
| Redundancy | 14 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 34 - 50 % (v/v) PEE, 0.1 M Hepes |
