5L9R

HIF prolyl hydroxylase 2 (PHD2/ EGLN1) in complex with N-oxalylglycine (NOG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41
Unit cell lengths	71.233, 71.233, 48.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.950 - 1.810
R-factor	0.1577
Rwork	0.156
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bqy
RMSD bond length	0.005
RMSD bond angle	0.776
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.945	1.870
High resolution limit [Å]	1.806	1.810
Rmerge	0.106	0.977
Number of reflections	22342
<I/σ(I)>	18.372	1.875
Completeness [%]	99.9	99.1
Redundancy	7.3	6.7
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES-Na pH 7.5, 2% v/v Polyethylene glycol 400, 2.0 M ammonium sulfate