5L8E
Structure of UAF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91997 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 73.303, 131.601, 148.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.300 |
| R-factor | 0.18731 |
| Rwork | 0.185 |
| R-free | 0.22426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.235 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 1.000 |
| Number of reflections | 64580 | |
| <I/σ(I)> | 16.1 | 1.4 |
| Completeness [%] | 99.6 | 95.9 |
| Redundancy | 4.5 | 4.1 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 200mM Tri-Sodium Citrate, Bis-Tris Propane Cryo -30% Glycerol |
