5L83
Complex of potato ATG8 protein with a peptide from Irish potato famine pathogen effector protein PexRD54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 172.180, 172.180, 172.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.090 - 1.900 |
| R-factor | 0.17604 |
| Rwork | 0.175 |
| R-free | 0.19994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.090 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 32648 | |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 32.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M-ammonium sulphate, 0.1M Tris buffer, pH 8.0 and 36 % PEG3350 |
