5L6W

Structure Of the LIMK1-ATPgammaS-CFL1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 32 2 1
Unit cell lengths	80.679, 80.679, 237.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.940 - 2.530
R-factor	0.22889
Rwork	0.226
R-free	0.28395
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q8g 3s95
RMSD bond length	0.015
RMSD bond angle	1.846
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.940
High resolution limit [Å]	2.530
Rmerge	0.061
Number of reflections	30888
<I/σ(I)>	19.1
Completeness [%]	100.0
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	35% pentaerythritol propoxylate 5/4, 0.1 M HEPES pH 7.5, 0.2 M potassium chloride