5L36
Crystal Structure of a human FasL mutant in complex with human DcR3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-01-11 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | H 3 2 |
Unit cell lengths | 127.267, 127.267, 206.262 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 3.100 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4msv |
RMSD bond length | 0.018 |
RMSD bond angle | 2.000 |
Data reduction software | iMOSFLM |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.200 | 0.735 |
Number of reflections | 11951 | |
<I/σ(I)> | 9.5 | 3.33 |
Completeness [%] | 98.9 | 100 |
Redundancy | 6.2 | 6.4 |
CC(1/2) | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M Sodium Acetate:Acetic Acid pH4.5; 1M Ammonium Phosphate Dibasic |