5L36
Crystal Structure of a human FasL mutant in complex with human DcR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-11 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 127.267, 127.267, 206.262 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 3.100 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4msv |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.000 |
| Data reduction software | iMOSFLM |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.200 | 0.735 |
| Number of reflections | 11951 | |
| <I/σ(I)> | 9.5 | 3.33 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 6.2 | 6.4 |
| CC(1/2) | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M Sodium Acetate:Acetic Acid pH4.5; 1M Ammonium Phosphate Dibasic |
