5L2R
Crystal structure of fumarate hydratase from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.675, 85.128, 241.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 120.738 - 2.054 |
R-factor | 0.1451 |
Rwork | 0.144 |
R-free | 0.17730 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.114 | 0.410 |
Number of reflections | 83691 | |
<I/σ(I)> | 16.5 | 2.8 |
Completeness [%] | 98.1 | 97.9 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350 and tacsimate pH5 |