5L1X
Structure of the Human Metapneumovirus Fusion Protein in the Postfusion Conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 128.720, 128.720, 572.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.019 - 3.300 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rrr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.123 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.020 | 37.020 | 3.370 |
| High resolution limit [Å] | 3.300 | 16.170 | 3.300 |
| Rmerge | 0.216 | 0.053 | 1.015 |
| Rmeas | 0.238 | ||
| Rpim | 0.099 | ||
| Total number of observations | 398399 | ||
| Number of reflections | 73617 | ||
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 99.8 | 89.2 | 99.9 |
| Redundancy | 5.4 | 4.1 | 5.6 |
| CC(1/2) | 0.993 | 0.998 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 18.5% PEG3350, 11% MPD, 0.2 M lithium sulfate, 0.01 M nickel chloride, 0.1 M imidazole |
