5L1X

Structure of the Human Metapneumovirus Fusion Protein in the Postfusion Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	128.720, 128.720, 572.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.019 - 3.300
R-factor	0.223
Rwork	0.221
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rrr
RMSD bond length	0.008
RMSD bond angle	1.123
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.12)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.020	37.020	3.370
High resolution limit [Å]	3.300	16.170	3.300
Rmerge	0.216	0.053	1.015
Rmeas	0.238
Rpim	0.099
Total number of observations	398399
Number of reflections	73617
<I/σ(I)>	5.2
Completeness [%]	99.8	89.2	99.9
Redundancy	5.4	4.1	5.6
CC(1/2)	0.993	0.998	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	18.5% PEG3350, 11% MPD, 0.2 M lithium sulfate, 0.01 M nickel chloride, 0.1 M imidazole