5L1E

AMPA subtype ionotropic glutamate receptor GluA2 in complex with noncompetitive inhibitor CP465022

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	92.677, 109.858, 602.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.016 - 4.370
R-factor	0.2415
Rwork	0.239
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kg2
RMSD bond length	0.002
RMSD bond angle	0.539
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.370	4.370
Rmerge	0.102	1.502
Number of reflections	75334
<I/σ(I)>	8.15
Completeness [%]	98.0
Redundancy	4.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11-14% (w/v) PEG 6,000, 0.1 M ammonium phosphate and 0.1 M TRIS (pH 7.9-8.0)