5L0P

Symmetry-based assembly of a two-dimensional protein lattice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	131.313, 54.833, 88.480
Unit cell angles	90.00, 128.47, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.198
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1JI7 & 2FU4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge		0.283
Number of reflections	20782
<I/σ(I)>	15.9
Completeness [%]	93.9
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	100 mM Tris, pH 8.0, 3.5 M sodium chloride