5L0O
IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.752, 72.464, 73.272 |
| Unit cell angles | 77.15, 72.07, 86.80 |
Refinement procedure
| Resolution | 15.000 - 2.360 |
| R-factor | 0.262 |
| Rwork | 0.262 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i6x |
| Data reduction software | SAINT |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.060 | 2.460 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.085 | 0.350 |
| Number of reflections | 41528 | |
| <I/σ(I)> | 25.57 | 2.14 |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 3.13 | 1.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 18% PEG 3350, 10% ethylene glycol, 10% glucose, 100mM -160mM potassium acetate |
