5L0J
Human vinculin R903Q, D907R, R910T mutant(residues 891-1066)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.430, 86.430, 175.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.790 - 4.000 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rke |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 175.200 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rpim | 0.043 | |
| Number of reflections | 6727 | |
| <I/σ(I)> | 13.3 | 2.9 |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 5.9 | 5.6 |
| CC(1/2) | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M HEPES sodium (pH 7.5), and 1.5 M Lithium sulfate monohydrate |
