5L0J
Human vinculin R903Q, D907R, R910T mutant(residues 891-1066)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 86.430, 86.430, 175.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.790 - 4.000 |
R-factor | 0.293 |
Rwork | 0.293 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rke |
RMSD bond length | 0.010 |
RMSD bond angle | 1.180 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 175.200 | 4.220 |
High resolution limit [Å] | 4.000 | 4.000 |
Rpim | 0.043 | |
Number of reflections | 6727 | |
<I/σ(I)> | 13.3 | 2.9 |
Completeness [%] | 98.7 | 99.3 |
Redundancy | 5.9 | 5.6 |
CC(1/2) | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M HEPES sodium (pH 7.5), and 1.5 M Lithium sulfate monohydrate |