5L0H
Human metavinculin MVt cardiomyopathy-associated mutant R975W (residues 959-1134) in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 80.951, 80.951, 131.762 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.110 - 2.900 |
R-factor | 0.2559 |
Rwork | 0.255 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3myi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.110 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rpim | 0.018 | |
Number of reflections | 6145 | |
<I/σ(I)> | 43.5 | 6.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 40.8 | 42.5 |
CC(1/2) | 0.983 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.2 M LiSO4, 0.1 M HEPES (pH 7.5), and 22% PEG 3350 |