5L0D
Human Metavinculin(residues 959-1130) in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-11 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.617, 58.550, 112.492 |
Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
Resolution | 50.130 - 2.750 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3myi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rpim | 0.048 | |
Number of reflections | 19048 | |
<I/σ(I)> | 16.3 | 8.2 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 8.7 | 8.6 |
CC(1/2) | 0.995 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.4 | 294 | 20 mM citric acid, 80-100 mM Bis-Tris propane (pH 3.4), and 12-16% (w/v) PEG 3350 |