5L04
STRUCTURE OF INTERFERON LAMBDA 1 RECEPTOR WITH HUMAN KINASE JAK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-02 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.700, 53.800, 87.390 |
Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
Resolution | 79.380 - 2.100 |
R-factor | 0.1707 |
Rwork | 0.169 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.852 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.000 | 99.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.077 | 0.050 | 0.634 |
Rmeas | 0.081 | ||
Rpim | 0.025 | ||
Total number of observations | 384056 | ||
Number of reflections | 40748 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 98.6 | 99.7 | 89.7 |
Redundancy | 9.4 | 9.4 | 8 |
CC(1/2) | 0.998 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 7 mg/ml of protein in 50 mM Hepes buffer (pH 7.5) with 150 mM NaCl, mixesd with 1:1 ratio with 18% (w/v) PEG 3350, 0.2 M amonium formate |