5KYF

Crystal structure of USP7 catalytic domain [L299A] mutant in complex with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	62.490, 76.560, 88.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.127 - 1.450
R-factor	0.176
Rwork	0.176
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nbf
RMSD bond length	0.010
RMSD bond angle	1.057
Data reduction software	MOSFLM (7.1.1)
Data scaling software	Aimless (0.2.8)
Phasing software	PHASER (1.10.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.130	35.130	1.480
High resolution limit [Å]	1.450	7.810	1.450
Rmerge	0.078	0.026	0.823
Rmeas	0.084
Rpim	0.031
Total number of observations	527391
Number of reflections	74019
<I/σ(I)>	13.3
Completeness [%]	97.9	98.9	96
Redundancy	7.1	6.3	7.3
CC(1/2)	0.999	0.999	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.1M HEPES pH 7.5, 25% PEG3350