5KYF
Crystal structure of USP7 catalytic domain [L299A] mutant in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.490, 76.560, 88.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.127 - 1.450 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM (7.1.1) |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | PHASER (1.10.1) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.130 | 35.130 | 1.480 |
| High resolution limit [Å] | 1.450 | 7.810 | 1.450 |
| Rmerge | 0.078 | 0.026 | 0.823 |
| Rmeas | 0.084 | ||
| Rpim | 0.031 | ||
| Total number of observations | 527391 | ||
| Number of reflections | 74019 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 97.9 | 98.9 | 96 |
| Redundancy | 7.1 | 6.3 | 7.3 |
| CC(1/2) | 0.999 | 0.999 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M HEPES pH 7.5, 25% PEG3350 |
