5KYB
Crystal structure of the apo-form of USP7 catalytic domain [V302K] mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.620, 68.280, 77.060 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 56.719 - 2.200 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM (7.1.1) |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | PHASER (1.10.1) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.720 | 56.720 | 2.270 |
| High resolution limit [Å] | 2.200 | 9.070 | 2.200 |
| Rmerge | 0.054 | 0.027 | 0.541 |
| Rmeas | 0.065 | ||
| Rpim | 0.035 | ||
| Total number of observations | 131027 | ||
| Number of reflections | 39712 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.6 | 99.7 | 99.3 |
| Redundancy | 3.3 | 3.5 | 3.3 |
| CC(1/2) | 0.999 | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1% Tryptone, 0.05M HEPES Na pH 7.0, 20% PEG3350 |
