5KXI
X-ray structure of the human Alpha4Beta2 nicotinic receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2016-02-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 127.099, 132.633, 202.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.996 - 3.941 |
R-factor | 0.286 |
Rwork | 0.285 |
R-free | 0.30740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology model |
RMSD bond length | 0.003 |
RMSD bond angle | 0.745 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.010 |
High resolution limit [Å] | 3.940 | 3.940 |
Rmerge | 0.137 | |
Rpim | 0.044 | 0.716 |
Number of reflections | 30759 | |
<I/σ(I)> | 14.9 | 1.1 |
Completeness [%] | 99.5 | 97.8 |
Redundancy | 9.1 | 7.5 |
CC(1/2) | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 0.05 M ADA pH 6.8, 12.5% PEG 1500 and 10% PEG 1000 |