5KWA
complete structure of the Mycobacterium tuberculosis proteasomal ATPase Mpa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 111.931, 111.931, 196.072 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.450 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.112 | 0.703 |
Number of reflections | 32237 | |
<I/σ(I)> | 7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 | 10.5 |
CC(1/2) | 0.984 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 288 | 100mM Tris-HCl, 20%PEG400, 200mM MgCl2, 5mM ATPrS |