5KVY

CRYSTAL STRUCTURE OF THE TWO TANDEM RRM DOMAINS OF PUF60 BOUND TO A PORTION OF AN ADML PRE-MRNA 3' SPLICE SITE ANALOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 31
Unit cell lengths	62.438, 62.438, 83.332
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.950
Rwork	0.196
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfj
RMSD bond length	0.006
RMSD bond angle	1.340
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.059	0.452
Number of reflections	26419
<I/σ(I)>		2.2
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	293	0.1 M TRIS-HCl, 25% PEG 4000, 5-10 mM Barium Chloride Dihydrate, pH 8.7, mixed with 10 mg/ml protein-nucleic acid mixture in 50 mM TRIS-HCl, pH 8.0, 150 mM NaCl, 20 micromolar EDTA.