5KUY
Influenza hemagglutinin H3 A/Hong Kong/1/1968 in complex with designed inhibitor protein HSB.2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.259, 219.322, 104.524 |
| Unit cell angles | 90.00, 117.78, 90.00 |
Refinement procedure
| Resolution | 48.134 - 2.598 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fnk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.134 | 2.690 |
| High resolution limit [Å] | 2.598 | 2.600 |
| Rmerge | 0.586 | |
| Number of reflections | 95012 | |
| <I/σ(I)> | 13.3 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.4 | 5.3 |
| CC(1/2) | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 10% PEG 6000, 100 mM MES pH 6.0 |
