5KUM
Crystal Structure of Inward Rectifier Kir2.2 K62W Mutant In Complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2015-04-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | I 4 |
Unit cell lengths | 81.303, 81.303, 183.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.950 - 2.800 |
R-factor | 0.20051 |
Rwork | 0.198 |
R-free | 0.25823 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kuk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.444 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.950 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.092 | 0.743 |
Number of reflections | 14556 | |
<I/σ(I)> | 11.65 | 1.67 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293.15 | Hepes, KCl, PEG 400 |