5KUM
Crystal Structure of Inward Rectifier Kir2.2 K62W Mutant In Complex with PIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2015-04-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 81.303, 81.303, 183.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.950 - 2.800 |
| R-factor | 0.20051 |
| Rwork | 0.198 |
| R-free | 0.25823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kuk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.950 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.092 | 0.743 |
| Number of reflections | 14556 | |
| <I/σ(I)> | 11.65 | 1.67 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | Hepes, KCl, PEG 400 |
