5KUJ

Human mitochondrial calcium uniporter (residues 72-189) crystal structure with magnesium.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	55.360, 55.360, 68.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.943 - 1.600
R-factor	0.1753
Rwork	0.173
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kue
RMSD bond length	0.010
RMSD bond angle	1.201
Data reduction software	iMOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	68.980
High resolution limit [Å]	1.600
Rmerge	0.085
Number of reflections	15893
<I/σ(I)>	17.6
Completeness [%]	99.9
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.32 M magnesium chloride, 0.1 M Tris pH 8.5, 24 % PEG 400, 1.6 M ammonium sulfate.