5KUI

Human mitochondrial calcium uniporter (residues 72-189) crystal structure with calcium.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-18
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	51.280, 51.280, 71.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.756 - 2.701
R-factor	0.2804
Rwork	0.273
R-free	0.29950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kue
RMSD bond length	0.004
RMSD bond angle	0.620
Data reduction software	iMOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.710
High resolution limit [Å]	2.700
Rmerge	0.086
Number of reflections	2858
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M calcium acetate, 0.1 M imidazole pH 8.0, 10 % PEG 8000, 1.3 M ammonium sulfate.