5KTY

hMiro EF hand and cGTPase domains, GDP and Ca2+ bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	74.073, 74.073, 222.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.174 - 2.522
R-factor	0.2133
Rwork	0.209
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0l
RMSD bond length	0.004
RMSD bond angle	0.675
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.100
Number of reflections	21762
<I/σ(I)>	23.6
Completeness [%]	97.3
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	10 mg/mL protein, 5 mM calcium chloride, 1 mM GDP, 0.04 M potassium phosphate, 16% w/v PEG8000, 20% v/v glycerol