5KTY
hMiro EF hand and cGTPase domains, GDP and Ca2+ bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.073, 74.073, 222.175 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.174 - 2.522 |
R-factor | 0.2133 |
Rwork | 0.209 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c0l |
RMSD bond length | 0.004 |
RMSD bond angle | 0.675 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | |
Number of reflections | 21762 | |
<I/σ(I)> | 23.6 | |
Completeness [%] | 97.3 | |
Redundancy | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 10 mg/mL protein, 5 mM calcium chloride, 1 mM GDP, 0.04 M potassium phosphate, 16% w/v PEG8000, 20% v/v glycerol |