5KT6
Teranry complex of human DNA polymerase iota(1-445) inserting dCMPNPP opposite template G in the presence of Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.269, 98.269, 201.656 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.738 - 3.540 |
R-factor | 0.2059 |
Rwork | 0.204 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.738 | 50.000 | 3.620 |
High resolution limit [Å] | 3.540 | 9.640 | 3.560 |
Rmerge | 0.222 | 0.050 | 0.990 |
Number of reflections | 7564 | ||
<I/σ(I)> | 3.3 | ||
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 14.9 | 16.1 | 14.4 |
CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate |