5KSZ

hMiro EF hand and cGTPase domains in the GMPPCP-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978720
Spacegroup name	P 43 21 2
Unit cell lengths	74.180, 74.180, 218.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.680 - 2.500
R-factor	0.2134
Rwork	0.211
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0l
RMSD bond length	0.004
RMSD bond angle	0.612
Data reduction software	MOSFLM (7.1.0)
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.249
Number of reflections	22095
<I/σ(I)>	10.1
Completeness [%]	100.0
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	12 mg/mL protein, 6 mM magnesium chloride, 10 mM GMPPCP, 0.2 M ammonium sulfate, 0.1 M trisodium citrate, pH 5.6, 17.5% w/v PEG4000