5KSU
Crystal structure of HLA-DQ2.5-CLIP1 at 2.73 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.860, 69.210, 146.690 |
| Unit cell angles | 90.00, 110.29, 90.00 |
Refinement procedure
| Resolution | 39.164 - 2.730 |
| R-factor | 0.1931 |
| Rwork | 0.190 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s9v |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.316 |
| Data reduction software | HKL-2000 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1137) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 2.800 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.100 | 0.998 |
| Number of reflections | 30494 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 93.7 | 86 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, ammonium sulphate, sodium cacodylate, glycerol |
