5KSE

Flap endonuclease 1 (FEN1) R100A with 5'-flap substrate DNA and Sm3+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 31 2 1
Unit cell lengths	105.235, 105.235, 104.064
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.997 - 2.105
R-factor	0.2096
Rwork	0.207
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2383: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.997	36.997	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.065	0.018	7.894
Number of reflections	73363
<I/σ(I)>	12.67	58.26	0.14
Completeness [%]	97.7	97.8	70.9
Redundancy	4.5
CC(1/2)	0.999	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	22% mPEG 2000, 20% saturated KCl, 5% ethylene glycol, 100 mM HEPES pH 7.5