5KQZ

Protease E35D-CaP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-11
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	46.060, 58.430, 86.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.200 - 1.700
Rwork	0.183
R-free	0.21500
Data reduction software	iMOSFLM (7.1.1)
Data scaling software	Aimless
Phasing software	PHASER (2.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	22.200
High resolution limit [Å]	1.700
Number of reflections	23936
<I/σ(I)>	16.7
Completeness [%]	99.0
Redundancy	6.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	sodium chloride