5KQ1

Crystal structure of S. pombe Dcp1/Dcp2 in complex with H. sapiens PNRC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 1 21 1
Unit cell lengths	45.622, 118.727, 93.174
Unit cell angles	90.00, 102.77, 90.00

Refinement procedure

Resolution	44.494 - 3.002
R-factor	0.212
Rwork	0.210
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkm
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	HKL-2000 (v708c)
Data scaling software	HKL-2000 (v708c)
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.106	0.061	0.745
Number of reflections	19125
<I/σ(I)>	10.3
Completeness [%]	99.0	96.2	91.9
Redundancy	3.7	3.7	3.4
CC(1/2)		0.990	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	150 mM trilithium citrate, 13% PEG 3350, 0.1% mellitic acid