5KPD
Mouse pgp 34 linker deleted double EQ mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.953, 116.594, 375.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.416 - 3.350 |
| R-factor | 0.2417 |
| Rwork | 0.240 |
| R-free | 0.28260 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ko2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (dev_2443) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.944 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.101 | 0.463 |
| Number of reflections | 62273 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 96.5 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | In the setup the rotein at 10.6 mg of 4 ul volume was diluted with 0.2 ul water and treated with 4 ul of 28% PEG400, 50 mM Glycine 9.0 and 100 mM NaCl. The reservoir solution conatined 100 mM Glycine 9.0pH, 150 mM NaCl, 10 mM NaN3 and 30% PEG400 |
