5KP8
Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Acetyl Donor-ACP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 100.892, 100.892, 104.461 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | |
Low resolution limit [Å] | 87.380 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.116 |
Number of reflections | 48869 |
<I/σ(I)> | 16.9 |
Completeness [%] | 100.0 |
Redundancy | 19.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 6% PEG 8000, 100 mM (NH4)2SO4, 1X MMT pH 6.5 |